Arthrosis |
The influence of irradiation with low-level
diode laser on the proteoglycan content in arthrotically
changed cartilage in rabbits
Tonio Gottlieba,
Björn Jörgensenb,
Ewa Rohdec,
Gerhard Müllerc,
Eike Eric Schellera,_
- a. Ev. Krankenhaus Hubertus,
Spanische Allee 10-14, 14129 Berlin, Germany
- b. Endozentrum Martin Luther
Krankenhaus, Caspar-Theyß-Str. 27-31, 14193,
Berlin, Germany cInstitut fu¨r
Medizinische Physik und Lasermedizin,
Charite´ Campus Benjamin Franklin, Fabeckstr.
60-62, 14195, Berlin, Germany
Received 24 November 2005; accepted 16 December 2005
Abstract
The course of arthrosis was investigated on an
animal-experimental arthrosis model considering macroscopic
aspects, and the proteoglycan and the glycosaminoglycan
contents. Based on these parameters, the influence of a low
power diode laser of 692.6nm wavelength on the progress of
arthrosis was investigated. Thirty days following joint
instability surgery another operation was made during which
the femoral condyles were irradiated using different energy
densities. Seven days after the second operation,
macroscopic findings were made and the proteoglycan content
was established based on the quantitative determination
according to Taylor and Jeffre. This method is based on
various spectrophotometric absorption behaviours of
different concentrations of sulphatized glycosaminoglycans
in the presence of dimethylmethylene blue.
Macroscopically, a
progressively increasing severity of cartilage changes
during the course of arthrosis was detected and the
proteoglycan content was found to decrease. The changes in
the irradiated joints proved to be less severe, with the
higher energy density having a greater positive influence of
statistical significance.
r 2006 Elsevier GmbH. All rights
reserved. Keywords: Cartilage; Laser; Proteoglycans;
Spectrophotometry
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