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Quantitative and qualitative changes
of the seminiferous epithelium induced by Ga. Al.
As.
(830 nm) laser radiation.
Taha MF; Valojerdi MR
Lasers in surgery and medicine; VOL: 34 (4);
p. 352-9 /2004/
Department of Anatomy, School of Medical Sciences,
Tarbiat Modarres University, P.O. Box 14115-111,
Tehran, Iran.
BACKGROUND AND OBJECTIVES: Low level laser radiation
stimulates both nucleic acid synthesis and cellular
proliferation in E. coli, Hela tumor cells,
fibroblasts, lymphocytes, and thyroid cells. It has
been introduced as a therapeutic modality;
nevertheless few studies have been carried out to
determine the effects of laser radiation on the
testes or spermatogenesis. The aim of this study was
to determine the quantitative and qualitative
changes of the seminiferous epithelium after Ga. Al.
As. (830 nm) laser radiation.
STUDY DESIGN/MATERIALS AND METHODS: The left testes
of Sprague-Dawley rats were daily exposed to laser
light for 15 days; so the cumulative doses used
28.05 and 46.80 J/cm(2) in two experimental groups.
Sampling carried out 24 hours after the last
treatment and samples were processed for LM and TEM
study.
RESULTS: The number of germ cells specially the
pachytene spermatocytes and elongated spermatids
increased after 28.05 J/cm(2) laser radiation.
Ultrastructural features of germ and Sertoli cells
in this group were similar to that of control; while
laser irradiation at 46.80 J/cm(2) had a destructive
effect on the seminiferous epithelium such as
dissociation of immature spermatids and evident
ultrastructural changes in them.
CONCLUSIONS: The findings confirmed the existence of
a biostimulatory threshold of applied laser energy
and the importance of determining it for clinical
applications. Moreover, it was revealed that low
doses of laser light have a biostimulatory effect on
the spermatogenesis and may provide benefits to the
patients with oligospermia and azoospermia. |
